A Secret Weapon For attune flow cytometer
A Secret Weapon For attune flow cytometer
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Data-enriched characterization of cell populations. Morphological facts from photographs can incorporate on the richness of flow cytometry data. One example is, the determine reveals an if not typical apoptosis assay applying Annexin V and PI, including cell imaging to characterize cells in Just about every populace to expose morphologically distinct characteristics. These insights could not have already been acquired from multiplex staining alone.
Whether or not you’re a beginner or an expert, discover our methods to progress your study and knowledge in flow cytometry.
(B) RAW 264.7 macrophages are frequently annotated inaccurately with regard to particle rely due to their complexity and noncircular borders. The amount of singlets readily available for Evaluation will increase once the user trains and employs a far more exact model.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing that has a high-speed brightfield camera for simultaneous superior throughput flow cytometry and substantial resolution brightfield imaging.
*Pricing is indicative only which is issue to change. Please Call your Agilent consultant with any questions. The data established forth herein is not really a proposal or even a commitment, each of which might be subject matter to credit history approval, conditions and terms, and availability with Agilent. Agilent Fiscal Methods is on the market in selected countries.
Additionally, it really is illuminating to view the number of doublets slip by traditional singlet gates, and terrific to validate the standing of unexpected double-positive occasions in my Evaluation."
Pair your Attune flow cytometer with the autosampler for amplified workflow performance, and include robotic integration for optimum throughput.
Our experts will structure panels to suit your needs as persistently you need—totally free, no product or service acquire wanted.
The Attune NxT cytometer may be the trustworthy workhorse instrument your lab can count on for constant and trustworthy general performance. The Attune CytPix design includes a large-speed brightfield digital camera and AI-driven image Examination software, with latest updates which include machine Discovering instruments and an intuitive consumer interface, enabling Increased facts precision, accuracy, and speedier insights into sample biology.
Customers can see occasion visuals in true-time to verify sample excellent, optimize protocols, and report the morphology of each cell populace determined for analysis. Imaging seize also Positive aspects from acoustic focusing, which helps to placement the cells so that a pointy, centered image is attained.
Seem waves help precise mobile alignment, causing Extraordinary info quality and ~10X faster sample flow charges.
We Earlier demonstrated the power of imaging Car or truck-T/Ramos cell interactions. Enable’s examine just the population of biggest interest, the double good occasions, to learn more. We are able to now use prolonged morphological parameters (right here, circularity and skewness of intensity) to further more analyze the attributes of those populations and refine gating on these events, raising knowledge robustness.
Designed-in dimensionality reduction applications amplified resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to verify envisioned population resolution in lysed full human blood. Consumer can perspective activities from Just about every cluster for visual affirmation of cell phenotypes.
No wash/no lyse leukocyte analysis with imaging. Cells have been obtained from 24-hour-previous blood by dilution in one mM EDTA ( Pro UX/UI enables buyers to coach AI products for expanded apps. (A) The “Prepare Product” attribute gives annotation tools and actual-time design training precision.
Gating on these extended parameters lets you rapidly and accurately recognize populations of desire and ensure gating technique with little if any guide evaluation. The Attune computer software gives entry to point out-of-the-artwork machine Studying Investigation resources inside of an expertly made consumer interface, rendering it exceptionally user friendly.
The Attune Xenith cytometer is usually a superior-parameter spectral analyzer, read more providing each spectral unmixing and traditional flow capabilities for thorough and versatile mobile Assessment.
Agilent Worth Guarantee guarantees you a minimum of a decade of instrument use within the date you purchase. Normally, We'll credit score you Along with the residual worth of the technique towards an upgraded design.
What seemed to be a homogeneous populace is really extra varied—an insight that needs to be regarded as when interpreting effects.